Journal
/
发育生物学
/
Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

DOI:
10.3791/55837-v

•

05:50 min

•

June 13, 2017

•

, , ,
1Department of Rehabilitation Sciences,University of Kentucky, 2Department of Physiology,University of Kentucky

Chapters

  • 00:05Title
  • 01:10Osmotic Pump Implantation
  • 02:49Direct Neurotrophin 4 (NTF4) Injection and Visualization
  • 04:42Results: Representative Effects of NTF4 Administration on Rat Laryngeal Muscle Fiber Size, Innervation, and Neuromuscular Junction Formation
  • 05:09Conclusion

Summary

自动翻译

自动翻译

Here, we present a protocol to describe the use of neurotrophin 4 (NTF4) systemically and directly to remodel rat aging laryngeal muscles.

Tags

SubcutaneousNeurotrophin 4Osmotic PumpsMuscular InjectionAgingLaryngeal MusclesVocal FunctionMuscle RemodelingMuscle AtrophyOtolaryngologySurgical ProcedureAseptic TechniqueAnesthesiaRat Model

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method

Induction and Validation of Cellular Senescence in Primary Human Cells

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Read Article