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내부 기준에 FACS 및 qPCR에 사용 단일 세포 유전자 발현 프로파일 링
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February 25, 2017
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We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.
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Porter, J. R., Telford, W. G., Batchelor, E. Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards. J. Vis. Exp. (120), e55219, doi:10.3791/55219 (2017).
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