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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
 

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

Article DOI: 10.3791/54677-v • 07:19 min • October 18th, 2016
October 18th, 2016 •
1, 1, 2, 1, 1,3, 1
1Department of Cell and Molecular Biology, The Scripps Research Institute, 2Department of Chemical Physiology, The Scripps Research Institute, 3The Lowy Medical Research Institute

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Microglial phagocytosis is critical for the maintenance of tissue homeostasis and inadequate phagocytic function has been implicated in pathology. However, assessing microglia function in vivo is technically challenging. We have developed a simple but robust technique for precisely monitoring and quantifying the phagocytic potential of microglia in a physiological setting.

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Retinal Microglial Phagocytic Function Flow Cytometry-based Assay Ophthalmology Compounds Microglia Phagocytic Function Quantitative Analysis Fluorescently Labeled Particles Anesthetized Mouse Surgical Microscope Sedation Eyelid Pressure Eye Ball Socket Intravitreal Injections Biased Results Variable Results Trauma To The Eye
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