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Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
Journal JoVE
Biologie
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Journal JoVE Biologie
Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

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07:13 min

August 16, 2016

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07:13 min
August 16, 2016

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Summary

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Herein we describe a sensitive immunochemical method for mapping the spatial distribution of 5mC oxidation derivatives based on the use of peroxidase-conjugated secondary antibodies and tyramide signal amplification.

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