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Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
JoVE 杂志
生物学
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JoVE 杂志 生物学
Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

DOI:
10.3791/54416-v

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07:13 min

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August 16, 2016

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, , , ,
1Laboratoire de Neurophysiologie (CP601), ULB Neuroscience Institute (UNI),Université Libre de Bruxelles, 2Medical Molecular Sciences, Centre for Biomolecular Sciences,University of Nottingham, 3School of Life Sciences,University of Nottingham, 4Division of Cancer and Stem Cells, Centre for Biomolecular Sciences, School of Medicine,University of Nottingham

Chapters

  • 00:05Title
  • 00:47Fixation and Permeabilization of Cryo- and Microtome Sections
  • 01:34Immunostaining for Oxi-5mC Derivatives
  • 05:16Results: Immunohistochemical Analysis of 5hmC and 5caC Distributions in the Adult Mouse Brain Tissues
  • 06:12Conclusion

Summary

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Herein we describe a sensitive immunochemical method for mapping the spatial distribution of 5mC oxidation derivatives based on the use of peroxidase-conjugated secondary antibodies and tyramide signal amplification.

Tags

Modified CytosineImmunohistochemistry5hmC5caCPeroxidase Conjugated Secondary AntibodiesTyramide Signal AmplificationNuclear LocalizationProtein Lineage MarkersDNA DepurinationPFAFAPBXPBTHClTris-HClBlocking SolutionMouse Monoclonal Anti-5hmCRabbit Polyclonal Anti-5caC

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