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Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
JoVE Revista
Biología
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JoVE Revista Biología
Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

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07:13 min

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August 16, 2016

DOI:

10.3791/54416-v

DOI:
10.3791/54416-v

•

07:13 min
August 16, 2016

•

1 Views
, , , ,
1Laboratoire de Neurophysiologie (CP601), ULB Neuroscience Institute (UNI),Université Libre de Bruxelles, 2Medical Molecular Sciences, Centre for Biomolecular Sciences,University of Nottingham, 3School of Life Sciences,University of Nottingham, 4Division of Cancer and Stem Cells, Centre for Biomolecular Sciences, School of Medicine,University of Nottingham

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Herein we describe a sensitive immunochemical method for mapping the spatial distribution of 5mC oxidation derivatives based on the use of peroxidase-conjugated secondary antibodies and tyramide signal amplification.

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Abakir, A., Wheldon, L., Johnson, A. D., Laurent, P., Ruzov, A. Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry. J. Vis. Exp. (114), e54416, doi:10.3791/54416 (2016).

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