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全山解剖和成年小鼠耳蜗的免疫
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Neurosciences
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Journal JoVE Neurosciences
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
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全山解剖和成年小鼠耳蜗的免疫

52,854 Views

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12:02 min

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January 01, 2016

DOI:

10.3791/53561-v

DOI:
10.3791/53561-v

•

12:02 min
January 01, 2016

•

56 Views
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1Department of Surgery, Division of Otolaryngology,Southern Illinois University, School of Medicine, 2Department of Pharmacology,Southern Illinois University, School of Medicine

Summary

Automatically generated

我们提出了一个方法，以分离科尔蒂的成年器官三个完整耳蜗匝（先端，中间，和碱）。我们还演示了一个程序，用荧光标记的抗体免疫染色。连同这些技术允许毛细胞，支持细胞，以及其他细胞类型中的耳蜗研究发现。

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Cite this Article

Montgomery, S. C., Cox, B. C. Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea. J. Vis. Exp. (107), e53561, doi:10.3791/53561 (2016).

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