TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Benjamin Bertin; Yoan Renaud; Rajaguru Aradhya; Krzysztof Jagla; Guillaume Junion

doi:10.3791/52985

Journal

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Biologie

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TRAP-RC，翻译核糖体亲和纯化的稀有细胞群果蝇胚胎

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Biologie

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Journal JoVE Biologie

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

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TRAP-RC，翻译核糖体亲和纯化的稀有细胞群果蝇胚胎

17,490 Views

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10:26 min

•

September 10, 2015

DOI:

10.3791/52985-v

DOI:

10.3791/52985-v

•

10:26 min

September 10, 2015

•

3 Views

Benjamin Bertin¹, Yoan Renaud¹, Rajaguru Aradhya², Krzysztof Jagla¹, Guillaume Junion¹

¹University of Clermont-Ferrand, ²Memorial Sloan Kettering Cancer Center

Summary

Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.

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TRAP Translating Ribosome Affinity Purification Rare Cell Populations Drosophila Embryos MRNA Translation Cell-type-specific Translatome Analysis Polysomes Tagged Ribosomal Subunit Developmental Processes Disease Progression Cell-specific Gene Expression Microarray RNA-seq Gal4/UAS System Muscle Cells

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TRAP-RC，翻译核糖体亲和纯化的稀有细胞群<em>果蝇</em>胚胎

Summary

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