Journal
/
Neurosciences
/
Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

DOI:
10.3791/51240-v

•

06:18 min

•

April 03, 2014

•

, ,
1Program in Neuroscience, Graduate School of Biomedical Sciences,University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry,University of Massachusetts Medical School

Chapitres

  • 00:05Titre
  • 01:17Prepare Brain Slices
  • 02:25Drug Treatment, Slice Biotinylation, and Tissue Lysate Preparation
  • 04:35Results: Biotinylation of Brain Slices
  • 05:55Conclusion

Summary

Traduction automatique

Traduction automatique

Neuronal membrane trafficking dynamically controls plasma membrane protein availability and significantly impacts neurotransmission. To date, it has been challenging to measure neuronal endocytic trafficking in adult neurons. Here, we describe a highly effective, quantitative method to measure rapid changes in surface protein expression ex vivo in acute brain slices.

Tags

Brain SliceBiotinylationEx VivoPlasma Membrane ProteinTraffickingAdult NeuronsEndocytic TraffickingSurface BiotinylationCultured CellsRegion-specificNeuromodulatory EventsReceptorIon ChannelTransporterCell Surface Presentation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

Using an α-Bungarotoxin Binding Site Tag to Study GABA A Receptor Membrane Localization and Trafficking

Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices

Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Read Article