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A Molecular Readout of Long-term Olfactory Adaptation in C. elegans
Chapitres
- 00:05Titre
- 01:57Cultivation and Maintenance of Animals for Nuclear Translocation Assays
- 03:26Long-term Odor Induced Nuclear Translocation Assays
- 05:44Scoring the Odor-induced Nuclear Translocation Event
- 08:54Results: Molecular Readout of Long-term Olfactory Adaptation in C. elegans
- 11:09Conclusion
Here we describe a molecular readout of long-term olfactory adaptation in Caenorhabditis elegans. The Protein Kinase G, EGL-4, is necessary for stable adaptation responses in the primary sensory neuron pair called AWC. During prolonged odor exposure EGL-4 translocates from the cytosol to nucleus of the AWC.
Tags
Molecular ReadoutLong-term Olfactory AdaptationC. ElegansSensory NeuronsAdaptation ResponseOlfactory CircuitGenetic ScreenMutantsVolatile OdorsAmphid Wing Cells Type C (AWC) Sensory NeuronsChemotaxis Behavioral AssayShort-term Olfactory AdaptationLong-term Olfactory AdaptationProtein Kinase G (PKG)EGL-4 Protein
