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Immunologie et infection
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Protein Misfolding Cyclic Amplification of Prions
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Immunologie et infection
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Journal JoVE Immunologie et infection
Protein Misfolding Cyclic Amplification of Prions

Protein Misfolding Cyclic Amplification of Prions

DOI:
10.3791/4075-v

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10:12 min

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November 07, 2012

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, ,
1Department of Civil Engineering,University of Nebraska at Lincoln, 2Department of Medical Microbiology and Immunology,Creighton University

Chapitres

  • 00:05Titre
  • 01:41Preparing the Samples
  • 03:13Amplification of Prion Strains using PMCA
  • 08:21Results: Western Blot of PK-digested HaCWD and DY TME Hamster-derived Prion Strains
  • 09:48Conclusion

Summary

Traduction automatique

Traduction automatique

Protein misfolding cyclic amplification (PMCA) is an in vitro assay for the study of prion conversion and strain and species barriers. It can also be used as a prion detection assay.

Tags

Protein MisfoldingCyclic AmplificationPrionsTransmissible Spongiform EncephalopathyPrPCPrPScIncubation PeriodClinical SignsAnimal Bioassay StudiesBiochemical PropertiesBiophysical PropertiesIn Vitro TechniqueSonicationIncubation CyclesPrPSc SeedSpecies SpecificityStrain SpecificityPrion Strain InterferenceAmplificationFluidsEnvironmental SamplesPMCA ProtocolContamination ControlConsistent ResultsQuantification

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