Journal
/
免疫与感染
/
Protein Misfolding Cyclic Amplification of Prions
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Protein Misfolding Cyclic Amplification of Prions

Protein Misfolding Cyclic Amplification of Prions

19,319 Views

•

10:12 min

•

November 07, 2012

DOI:

10.3791/4075-v

DOI:
10.3791/4075-v

•

10:12 min
November 07, 2012

•

7 Views
, ,
1Department of Civil Engineering,University of Nebraska at Lincoln, 2Department of Medical Microbiology and Immunology,Creighton University

Summary

Automatically generated

Protein misfolding cyclic amplification (PMCA) is an in vitro assay for the study of prion conversion and strain and species barriers. It can also be used as a prion detection assay.

Explore More Videos

Protein MisfoldingCyclic AmplificationPrionsTransmissible Spongiform EncephalopathyPrPCPrPScIncubation PeriodClinical SignsAnimal Bioassay StudiesBiochemical PropertiesBiophysical PropertiesIn Vitro TechniqueSonicationIncubation CyclesPrPSc SeedSpecies SpecificityStrain SpecificityPrion Strain InterferenceAmplificationFluidsEnvironmental SamplesPMCA ProtocolContamination ControlConsistent ResultsQuantification

Read Article

Cite this Article

Saunders, S. E., Bartz, J. C., Shikiya, R. A. Protein Misfolding Cyclic Amplification of Prions. J. Vis. Exp. (69), e4075, doi:10.3791/4075 (2012).

Download .ris file

Copy

Share Video

.

Copy