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Protein Misfolding Cyclic Amplification of Prions
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JoVE 杂志 免疫与感染
Protein Misfolding Cyclic Amplification of Prions

Protein Misfolding Cyclic Amplification of Prions

DOI:
10.3791/4075-v

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10:12 min

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November 07, 2012

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, ,
1Department of Civil Engineering,University of Nebraska at Lincoln, 2Department of Medical Microbiology and Immunology,Creighton University

Chapters

  • 00:05Title
  • 01:41Preparing the Samples
  • 03:13Amplification of Prion Strains using PMCA
  • 08:21Results: Western Blot of PK-digested HaCWD and DY TME Hamster-derived Prion Strains
  • 09:48Conclusion

Summary

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Protein misfolding cyclic amplification (PMCA) is an in vitro assay for the study of prion conversion and strain and species barriers. It can also be used as a prion detection assay.

Tags

Protein MisfoldingCyclic AmplificationPrionsTransmissible Spongiform EncephalopathyPrPCPrPScIncubation PeriodClinical SignsAnimal Bioassay StudiesBiochemical PropertiesBiophysical PropertiesIn Vitro TechniqueSonicationIncubation CyclesPrPSc SeedSpecies SpecificityStrain SpecificityPrion Strain InterferenceAmplificationFluidsEnvironmental SamplesPMCA ProtocolContamination ControlConsistent ResultsQuantification

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