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Immunologie et infection
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在实验性自身免疫性脑脊髓炎（EAE）抗性品系小鼠继转移和抗原挑战克服反应迟钝
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Immunologie et infection
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Journal JoVE Immunologie et infection
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
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在实验性自身免疫性脑脊髓炎（EAE）抗性品系小鼠继转移和抗原挑战克服反应迟钝

DOI:
10.3791/3778-v

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08:38 min

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April 09, 2012

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, ,
1Department of Medicine, Section of Cardiology,St. John-Providence Health System, 2Department of Immunology and Microbiology,Wayne State University School of Medicine

Chapitres

  • 00:05Titre
  • 01:28Preparing CFA-emulsified Antigen
  • 03:08Immunization of Donor Mice
  • 03:51Isolation of Lymph Node Cells after Immunization and Re-stimulation with Antigen
  • 06:07Harvest of Re-stimulated Lymph Node Cells and Adoptive Transfer
  • 06:47Antigenic Challenge of Recipient Mice and Development of Disease
  • 07:31Results: Kinetics of Disease Progression Following Immunization, Adoptive Transfer and Challenge
  • 07:58Conclusion

Summary

Traduction automatique

Traduction automatique

某些鼠标菌株能够抵抗与髓鞘碱性蛋白诱导实验性自身免疫性脑脊髓炎（EAE）。这里描述的是一个简单的免疫协议，扭转反应迟钝和麻痹性疾病引起几个典型的EAE的耐鼠标污渍。

Tags

Experimental Autoimmune EncephalomyelitisEAEMouse StrainsAdoptive TransferAntigenic ChallengeCentral Nervous SystemMononuclear CellsParalytic DiseaseMultiple SclerosisGenetic ControlC57BL/6 MiceMyelin Basic ProteinAntigen ProcessingAntigen PresentationEncephalitogenic T Cell ClonesCellular Adoptive Transfer System

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