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Utveckling av automatiserad bildbehandling och analys för zebrafisk kemiska skärmar.
Journal JoVE
Biologie
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Journal JoVE Biologie
Development of automated imaging and analysis for zebrafish chemical screens.
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Utveckling av automatiserad bildbehandling och analys för zebrafisk kemiska skärmar.

11,088 Views

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10:49 min

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June 24, 2010

DOI:

10.3791/1900-v

DOI:
10.3791/1900-v

•

10:49 min
June 24, 2010

•

1 Views
, , , ,
1Pharmacology and Chemical Biology,University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics,University of Pittsburgh, 3Department of Pharmaceutical Sciences,University of Pittsburgh, 4Department of Chemistry,University of Pittsburgh

Tags

Automated ImagingAnalysisZebrafish Chemical ScreensImage-based High-content ScreeningFGF/RAS/MAPK PathwayZebrafish EmbryosIn Vivo High-content Chemical Screens96-well PlatesHigh Throughput ScreeningAutomated Image Acquisition And AnalysisTransgenic Reporter LineGreen Fluorescent Protein (GFP)Whole Organism ScreensAutomated Imaging And Analysis24-48 Hours Post Fertilization (hpf)Multiwell PlatesSmall MoleculeHyperactivates FGF Signaling

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Vogt, A., Codore, H., Day, B. W., Hukriede, N. A., Tsang, M. Development of automated imaging and analysis for zebrafish chemical screens.. J. Vis. Exp. (40), e1900, doi:10.3791/1900 (2010).

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