Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Jianjun Zhong; Georgia Gunner; Nils Henninger; Dorothy P. Schafer; Daryl A. Bosco

doi:10.3791/64701

Journal

/

Neurociencias

/

使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

JoVE Revista
Neurociencias

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Revista Neurociencias

Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Please note that all translations are automatically generated. Click here for the English version.

使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

854 Views

•

08:25 min

•

April 21, 2023

DOI:

10.3791/64701-v

DOI:

10.3791/64701-v

•

08:25 min

April 21, 2023

•

3 Views

Jianjun Zhong^1,2, Georgia Gunner³, Nils Henninger¹, Dorothy P. Schafer³, Daryl A. Bosco¹

¹Department of Neurology,University of Massachusetts Chan Medical School, ²Department of Neurosurgery,The First Affiliated Hospital of Chongqing Medical University, ³Department of Neurobiology,University of Massachusetts Chan Medical School

Summary

这项研究表明，向小鼠提供重复性创伤性脑损伤，并同时植入颅窗，以便随后使用双光子显微镜对神经元表达的 EGFP 进行活体成像。

Explore More Videos

Intravital Imaging Fluorescent Protein Traumatic Brain Injury Cranial Window Two-photon Microscopy Neuronal Response Mechanical Stress Gene Of Interest Virus Promoter Cell Types Ophthalmic Ointment Midline Incision Periosteum Stereotaxic Frame TBI Impact Site Impactor Tip Dental Cement Lateral Muscles Cranial Window Implantation

Read Article

使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

Summary

Automatically generated

Explore More Videos