Journal
/
Bioquímica
/
Malachit-Green-Assay zur Entdeckung von Hitzeschockprotein-90-Inhibitoren
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
Please note that all translations are automatically generated. Click here for the English version.

Malachit-Green-Assay zur Entdeckung von Hitzeschockprotein-90-Inhibitoren

3,765 Views

•

07:57 min

•

January 20, 2023

DOI:

10.3791/64693-v

DOI:
10.3791/64693-v

•

07:57 min
January 20, 2023

•

47 Views
, , , , , ,
1Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), 2School of Life Sciences,University of Sussex

Summary

Automatically generated

Das Malachit-Green-Assay-Protokoll ist eine einfache und kostengünstige Methode zur Entdeckung von Hitzeschockprotein-90-Suppressoren (Hsp90) sowie anderer Hemmstoffe gegen ATP-abhängige Enzyme.

Explore More Videos

Malachite Green AssayHsp90ATPase ActivityPhosphate DetectionATP HydrolysisInhibitorsHigh-throughput ScreeningGeldanamycinSerial DilutionBlankPositive ControlAbsorbance

Read Article

Cite this Article

Gupta, S. D., Song, D., Lee, S., Lee, J. W., Park, J., Prodromou, C., Pan, C. Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors. J. Vis. Exp. (191), e64693, doi:10.3791/64693 (2023).

Download .ris file

Copy

Share Video

.

Copy