Journal
/
Bioquímica
/
Levende cellebilleddannelse af intakte ex vivo glober ved hjælp af en ny 3D-trykt holder
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

Levende cellebilleddannelse af intakte ex vivo glober ved hjælp af en ny 3D-trykt holder

2,460 Views

•

05:41 min

•

October 06, 2022

DOI:

10.3791/64510-v

DOI:
10.3791/64510-v

•

05:41 min
October 06, 2022

•

1 Views
, , , ,
1Department of Pharmacology, School of Medicine,Boston University, 2Department of Biochemistry, School of Medicine,Boston University, 3Department of Ophthalmology, School of Medicine,Boston University

Summary

Automatically generated

Det nuværende arbejde beskriver en ny eksperimentel protokol, der bruger en 3D-trykt holder til at muliggøre højopløsnings levende cellebilleddannelse af enukleerede kloder. Gennem denne protokol kan den cellulære calciumsignalaktivitet i såret hornhindeepitel fra ex vivo-kloder observeres i realtid.

Explore More Videos

3D Printed HolderLive-cell ImagingEx Vivo GlobesCorneal CellsEpitheliumNervesWound HealingCalcium IndicatorCell Membrane StainScratch WoundPuncture WoundCorneal Basement Membrane

Read Article

Cite this Article

Segars, K. L., Azzari, N. A., Gomez, S., Rich, C. B., Trinkaus-Randall, V. Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder. J. Vis. Exp. (188), e64510, doi:10.3791/64510 (2022).

Download .ris file

Copy

Share Video

.

Copy