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使用新型3D打印支架对完整的 离体 地球仪进行活细胞成像
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JoVE Revista Bioquímica
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
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使用新型3D打印支架对完整的 离体 地球仪进行活细胞成像

2,460 Views

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05:41 min

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October 06, 2022

DOI:

10.3791/64510-v

DOI:
10.3791/64510-v

•

05:41 min
October 06, 2022

•

1 Views
, , , ,
1Department of Pharmacology, School of Medicine,Boston University, 2Department of Biochemistry, School of Medicine,Boston University, 3Department of Ophthalmology, School of Medicine,Boston University

Summary

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这项工作描述了一种新的实验方案，该方案利用3D打印支架来实现去核球的高分辨率活细胞成像。通过该协议，可以实时观察离 体 球受伤角膜上皮中的细胞钙信号活性。

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Cite this Article

Segars, K. L., Azzari, N. A., Gomez, S., Rich, C. B., Trinkaus-Randall, V. Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder. J. Vis. Exp. (188), e64510, doi:10.3791/64510 (2022).

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