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微流体辅助轴突线粒体选择性去极化
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Neurociencias
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JoVE Revista Neurociencias
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
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微流体辅助轴突线粒体选择性去极化

1,577 Views

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06:55 min

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August 04, 2022

DOI:

10.3791/64196-v

DOI:
10.3791/64196-v

•

06:55 min
August 04, 2022

•

3 Views
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1TUM Medical Graduate Center,Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology,Technical University of Munich, 4Munich Cluster for Systems Neurology

Summary

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本协议描述了微流体室中神经元线粒体的播种和染色。这些腔室中的流体压力梯度允许选择性处理轴突中的线粒体，以分析其性质以应对药理学挑战而不影响细胞体区室。

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Cite this Article

Wanderoy, S., Rühmkorf, A., Harbauer, A. B. Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria. J. Vis. Exp. (186), e64196, doi:10.3791/64196 (2022).

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