/

/

使用双光镊和微流体进行单分子研究

JoVE Revista
Bioquímica

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Revista Bioquímica

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Please note that all translations are automatically generated. Click here for the English version.

使用双光镊和微流体进行单分子研究

1,897 Views

•

06:53 min

•

November 18, 2022

DOI:

10.3791/64023-v

DOI:

10.3791/64023-v

•

06:53 min

November 18, 2022

•

4 Views

Piero R. Bianco¹

¹Department of Pharmaceutical Sciences, College of Pharmacy,University of Nebraska Medical Center

Summary

使用玻璃桶、气密注射器、管道与流通池的稳定连接以及通过在注射器和管道之间放置切换阀来消除气泡，极大地促进了通过微流体室研究的视觉单分子生物化学。该协议描述了双光学陷阱，能够可视化DNA交易和分子间相互作用。

Explore More Videos

Dual Optical Tweezers Microfluidics Single-molecule Studies Flow Cell PTFE Tubing Syringe Pump Methanol Water Press-fit Tubing Connectors Microscope Slide

Read Article