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Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
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Neurociencias
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JoVE Revista Neurociencias
Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

DOI:
10.3791/63816-v

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09:00 min

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April 28, 2022

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, ,
1Department of Neurology,Northwestern University Feinberg School of Medicine

Capítulos

  • 00:04Introduction
  • 01:01Enrichment of SDS Insoluble Material
  • 03:29Amyloid Purification
  • 04:17Methanol Chloroform Precipitation
  • 04:58Trypsin Digestion
  • 06:02Peptide Cleanup
  • 07:12Results: Confirmation and Validation of Amyloid Purification
  • 08:14Conclusion

Summary

Traducción Automática

Traducción Automática

This biochemical purification method with mass spectrometry-based proteomic analysis facilitates the robust characterization of amyloid fibril cores, which may accelerate the identification of targets for preventing Alzheimer's disease.

Tags

Amyloid FibrilsAmyloid PlaquesAlzheimer’s DiseaseProtein PurificationProteomic CharacterizationHomogenizationSucrose GradientProtein AggregatesProtein Deposits

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