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Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
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神经科学
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JoVE 杂志 神经科学
Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

DOI:
10.3791/63816-v

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09:00 min

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April 28, 2022

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, ,
1Department of Neurology,Northwestern University Feinberg School of Medicine

Chapters

  • 00:04Introduction
  • 01:01Enrichment of SDS Insoluble Material
  • 03:29Amyloid Purification
  • 04:17Methanol Chloroform Precipitation
  • 04:58Trypsin Digestion
  • 06:02Peptide Cleanup
  • 07:12Results: Confirmation and Validation of Amyloid Purification
  • 08:14Conclusion

Summary

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This biochemical purification method with mass spectrometry-based proteomic analysis facilitates the robust characterization of amyloid fibril cores, which may accelerate the identification of targets for preventing Alzheimer's disease.

Tags

Amyloid FibrilsAmyloid PlaquesAlzheimer’s DiseaseProtein PurificationProteomic CharacterizationHomogenizationSucrose GradientProtein AggregatesProtein Deposits

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