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Analyse der Wechselwirkung fluoreszierend markierter Proteine mit künstlichen Phospholipid-Mikrovesikeln mittels quantitativer Durchflusszytometrie
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Analyse der Wechselwirkung fluoreszierend markierter Proteine mit künstlichen Phospholipid-Mikrovesikeln mittels quantitativer Durchflusszytometrie
1Center for Theoretical Problems of Physicochemical Pharmacology,Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics,Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics,Moscow Institute of Physics and Technology
Capítulos
- 00:04Introduction
- 00:58Kinetic Binding Experiments
- 02:13Equilibrium Binding Experiments
- 02:45Analysis of Flow Cytometry Data
- 05:13Converting Fluorescence Intensity to the Mean Number of Binding Sites
- 07:05Results: Specific Binding of Fluorescent-Labeled Coagulation Factor X or fX to Artificial Phospholipid Vesicles
- 07:42Conclusion
