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使用带有可更换盖子的乳腺成像窗口进行纵向活体腔内显微镜检查

JoVE Revista
Biología

This content is Free Access.

JoVE Revista Biología

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

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使用带有可更换盖子的乳腺成像窗口进行纵向活体腔内显微镜检查

2,695 Views

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10:53 min

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January 20, 2022

DOI:

10.3791/63326-v

DOI:

10.3791/63326-v

•

10:53 min

January 20, 2022

•

14 Views

Larissa Mourao^1,2, Marta Ciwinska^1,2, Jacco van Rheenen³, Colinda L. G. J. Scheele^1,2

¹Center for Cancer Biology,VIB (BE), ²Department of Oncology,KU Leuven (BE), ³Netherlands Cancer Institute, Department of Molecular Pathology,Oncode Institute (NL)

Summary

该协议描述了一种带有可更换盖子（R.MIW）的新型乳腺成像窗口。植入R.MIW后的活体显微镜检查允许在不同发育阶段以细胞分辨率对健康和患病的乳腺进行纵向和多日成像。

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