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使用微流体装置生产siRNA负载的脂质纳米颗粒
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
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使用微流体装置生产siRNA负载的脂质纳米颗粒

7,652 Views

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06:02 min

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March 22, 2022

DOI:

10.3791/62999-v

DOI:
10.3791/62999-v

•

06:02 min
March 22, 2022

•

48 Views
, , , , , ,
1Division of Applied Chemistry, Faculty of Engineering,Hokkaido University, 2JST PRESTO, 3Graduate School of Chemical Sciences and Engineering,Hokkaido University

Summary

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基于微流体的脂质纳米颗粒（LNP）生产方法在药物递送系统（DDS）中引起了人们的关注，包括RNA递送。该协议描述了使用我们名为iLiNP的原始微流体装置的制造，LNP（siRNA负载LNP）生产和LNP评估过程。

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Cite this Article

Maeki, M., Okada, Y., Uno, S., Niwa, A., Ishida, A., Tani, H., Tokeshi, M. Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device. J. Vis. Exp. (181), e62999, doi:10.3791/62999 (2022).

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