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Inmunología y contagio

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小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究

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Inmunología y contagio

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JoVE Revista Inmunología y contagio

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

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小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究

3,703 Views

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08:37 min

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February 15, 2021

DOI:

10.3791/62214-v

DOI:

10.3791/62214-v

•

08:37 min

February 15, 2021

•

16 Views

Gloria Delfanti¹, Alessandra Perini*^1,2, Elisa Zappa*^1,2, Maya Fedeli^1,2

¹Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases,IRCCS San Raffaele Scientific Institute, ²Vita-Salute San Raffaele University

Summary

我们描述了一种快速而强大的方案，以从小鼠脾脏中富集不变的自然杀伤T（iNKT）细胞，并在体外将其扩增至适合体外和体内研究的数量。

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