Journal
/
Biología del desarrollo
/
In vitro neuromuskulært veikryss indusert fra humane induserte pluripotente stamceller
JoVE Revista
Biología del desarrollo
This content is Free Access.
JoVE Revista Biología del desarrollo
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

In vitro neuromuskulært veikryss indusert fra humane induserte pluripotente stamceller

5,284 Views

•

06:01 min

•

December 03, 2020

DOI:

10.3791/61396-v

DOI:
10.3791/61396-v

•

06:01 min
December 03, 2020

•

5 Views
, , ,
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, 2Department of Pediatrics,Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine,Taipei Medical University

Summary

Automatically generated

Her gir vi en protokoll for å generere in vitro NMJs fra humane induserte pluripotente stamceller (iPSCer). Denne metoden kan indusere NMJs med moden morfologi og funksjon i 1 måned i en enkelt brønn. De resulterende NMJs kan potensielt brukes til å modellere relaterte sykdommer, for å studere patologiske mekanismer eller å screene narkotikaforbindelser for terapi.

Explore More Videos

Human Induced Pluripotent Stem CellsNeuromuscular JunctionMotor NeuronsSkeletal MuscleSchwann CellsSpinal Muscular AtrophyCell DifferentiationCell CultureCell DetachmentExtracellular MatrixDoxycyclineDifferentiation MediumMyogenic Differentiation MediumNeuromuscular Junction Medium

Read Article

Cite this Article

Lin, C., Yoshida, M., Li, L., Saito, M. K. In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (166), e61396, doi:10.3791/61396 (2020).

Download .ris file

Copy

Share Video

.

Copy