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从人类诱导多能干细胞诱导的体外神经肌肉结点

JoVE Revista
Biología del desarrollo

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JoVE Revista Biología del desarrollo

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

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从人类诱导多能干细胞诱导的体外神经肌肉结点

DOI:

10.3791/61396-v

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06:01 min

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December 03, 2020

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Chuang-Yu Lin, Michiko Yoshida², Li-Tzu Li, Megumu K. Saito

¹Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, ²Department of Pediatrics,Kyoto Prefectural University of Medicine, ³Graduate Institute of Clinical Medicine,Taipei Medical University

Capítulos

00:04Introduction
00:59Induced Pluripotent Stem Cell (iPSC) Culture
02:00iPSC Differentiation
03:04Neuromuscular Junction (NMJ) Muscle Contraction and Curare Treatment
03:47Results: Representative NMJ Phenotypic, Morphological, and Functional Characterization
05:20Conclusion

Summary

Traducción Automática

在这里，我们提供一个协议，从人类诱导多能干细胞（iPSCs）生成体外NMJ。该方法在1个月内可以诱导形态成熟、功能良好的NMJs。由此产生的NMJs可能用于模拟相关疾病、研究病理机制或筛选药物化合物以进行治疗。

Tags

Human Induced Pluripotent Stem Cells Neuromuscular Junction Motor Neurons Skeletal Muscle Schwann Cells Spinal Muscular Atrophy Cell Differentiation Cell Culture Cell Detachment Extracellular Matrix Doxycycline Differentiation Medium Myogenic Differentiation Medium Neuromuscular Junction Medium

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