Journal
/
Biología del desarrollo
/
从人类诱导多能干细胞诱导的体外神经肌肉结点
JoVE Revista
Biología del desarrollo
This content is Free Access.
JoVE Revista Biología del desarrollo
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
Please note that all translations are automatically generated. Click here for the English version.

从人类诱导多能干细胞诱导的体外神经肌肉结点

5,283 Views

•

06:01 min

•

December 03, 2020

DOI:

10.3791/61396-v

DOI:
10.3791/61396-v

•

06:01 min
December 03, 2020

•

5 Views
, , ,
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, 2Department of Pediatrics,Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine,Taipei Medical University

Summary

Automatically generated

在这里，我们提供一个协议，从人类诱导多能干细胞（iPSCs）生成体外NMJ。该方法在1个月内可以诱导形态成熟、功能良好的NMJs。由此产生的NMJs可能用于模拟相关疾病、研究病理机制或筛选药物化合物以进行治疗。

Explore More Videos

Human Induced Pluripotent Stem CellsNeuromuscular JunctionMotor NeuronsSkeletal MuscleSchwann CellsSpinal Muscular AtrophyCell DifferentiationCell CultureCell DetachmentExtracellular MatrixDoxycyclineDifferentiation MediumMyogenic Differentiation MediumNeuromuscular Junction Medium

Read Article

Cite this Article

Lin, C., Yoshida, M., Li, L., Saito, M. K. In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (166), e61396, doi:10.3791/61396 (2020).

Download .ris file

Copy

Share Video

.

Copy