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JoVE Journal Neuroscience
Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage
 

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

Article DOI: 10.3791/61322-v • 10:34 min • August 30th, 2020
August 30th, 2020 •
1, 1, 1,2, 1,2, 1
1University of Rouen Normandy, INSERM U1239, DC2N, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France, 2Department of Anesthesiology and Critical Care, Rouen University Hospital, Rouen, France

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We described in this protocol a standardized subarachnoid hemorrhage (SAH) mouse model by a double injection of autologous whole-blood into the cisterna magna. The high degree of standardization of the double-injection procedure represents a middle-to-acute model of SAH with relative safety regarding mortality.

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Double Direct Injection Blood Cisterna Magna Subarachnoid Hemorrhage Protocol Physiology Aneurysm Rupture Extravasation Subarachnoid Space Stroke Cases Mortality Rate Cognitive Sequelae Cerebral Ischemia Vasoconstriction Microscopic Vasospasm Therapeutic Options Pathological Events Animal Models Intercranial Aneurysm
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