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蛋白质组学设施提交和后续数据分析的TMT样品制备
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
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蛋白质组学设施提交和后续数据分析的TMT样品制备

12,066 Views

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07:44 min

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June 08, 2020

DOI:

10.3791/60970-v

DOI:
10.3791/60970-v

•

07:44 min
June 08, 2020

•

10 Views
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1Department of Pediatrics,University of Florida

Summary

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我们提出了优化的串联质量标签 （TMT） 标签协议，其中包括以下每个步骤的详细信息：蛋白质提取、定量、沉淀、消化、标记、提交到蛋白质组学设施以及数据分析。

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Cite this Article

Suzuki-Hatano, S., Tsai, A., Daugherty, A., Pacak, C. A. TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis. J. Vis. Exp. (160), e60970, doi:10.3791/60970 (2020).

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