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종양 내 이질성, 온코스트림 및 침략의 공간 및 분자 특성화를 위한 신경교종 소구역의 레이저 캡처 미세 분해
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Neurociencias
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JoVE Revista Neurociencias
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
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종양 내 이질성, 온코스트림 및 침략의 공간 및 분자 특성화를 위한 신경교종 소구역의 레이저 캡처 미세 분해

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09:09 min

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April 12, 2020

DOI:

10.3791/60939-v

DOI:
10.3791/60939-v

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09:09 min
April 12, 2020

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1 Views
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1Dept. of Neurosurgery,University of Michigan Medical School, 2Dept. of Cell and Developmental Biology,University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science,University of Michigan Medical School, 4Rogel Cancer Center,University of Michigan Medical School

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Laser MicrodissectionRNA SequencingIntratumor HeterogeneityMesenchymal CellsTumor InvasionTumor MorphologyMolecular ProfilesTumor ProgressionTherapeutic TargetsBioluminescencePerfusionCryopreservationSpatial CharacterizationMolecular Characterization

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Comba, A., Dunn, P. J., Kish, P. E., Kadiyala, P., Kahana, A., Castro, M. G., Lowenstein, P. R. Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion. J. Vis. Exp. (158), e60939, doi:10.3791/60939 (2020).

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