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Markerless Gene Deletion door Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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JoVE Revista Inmunología y contagio
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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Markerless Gene Deletion door Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

6,450 Views

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10:35 min

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January 30, 2020

DOI:

10.3791/60848-v

DOI:
10.3791/60848-v

•

10:35 min
January 30, 2020

•

8 Views
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1Department of Microbiology, Immunology, and Molecular Genetics,University of Kentucky

Summary

Automatically generated

Hier beschreven is een methode voor gerichte, markerless gen verwijdering in Chlamydia trachomatis met behulp van floxed cassette allelic uitwisseling mutagenese, FLAEM.

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Cite this Article

Keb, G., Fields, K. A. Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis. J. Vis. Exp. (155), e60848, doi:10.3791/60848 (2020).

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