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단백질-지질 상호작용을 측정하기 위한 마이크로스케일 열영동 사용
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Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
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단백질-지질 상호작용을 측정하기 위한 마이크로스케일 열영동 사용

5,988 Views

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04:45 min

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February 10, 2022

DOI:

10.3791/60607-v

DOI:
10.3791/60607-v

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04:45 min
February 10, 2022

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14 Views
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1Department of Biochemistry,University of Illinois at Urbana-Champaign, 2Department of Chemistry,University of South Florida, 3Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign

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Microscale ThermophoresisProtein-lipid InteractionsVam7Phosphatidic AcidDisassociation Constant (KD)Biochemical InteractionsNTA-Atto 647 DyeThermophoretic Traces1:1 TitrationDiC8 PAPharmaceutical Development

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Sparks, R. P., Lawless, W., Arango, A. S., Tajkhorshid, E., Fratti, R. A. Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions. J. Vis. Exp. (180), e60607, doi:10.3791/60607 (2022).

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