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Uso della termoforesi su microscala per misurare le interazioni proteina-lipidi

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

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Uso della termoforesi su microscala per misurare le interazioni proteina-lipidi

5,979 Views

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04:45 min

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February 10, 2022

DOI:

10.3791/60607-v

DOI:

10.3791/60607-v

•

04:45 min

February 10, 2022

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14 Views

Robert P. Sparks*¹, William Lawless*², Andres S. Arango*^1,3,4, Emad Tajkhorshid^3,4, Rutilio A. Fratti³

¹Department of Biochemistry,University of Illinois at Urbana-Champaign, ²Department of Chemistry,University of South Florida, ³Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign, ⁴Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign

Tags

Microscale Thermophoresis Protein-lipid Interactions Vam7 Phosphatidic Acid Disassociation Constant (KD)Biochemical Interactions NTA-Atto 647 Dye Thermophoretic Traces 1:1 Titration DiC8 PA Pharmaceutical Development

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