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エピジェネティックベースの定量PCRを用いた免疫細胞の同一性と純度の測定
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
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エピジェネティックベースの定量PCRを用いた免疫細胞の同一性と純度の測定

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08:02 min

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February 19, 2020

DOI:

10.3791/60465-v

DOI:
10.3791/60465-v

•

08:02 min
February 19, 2020

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1Cell Biology, Life Sciences Solutions,Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH,Precision for Medicine

Tags

Immune Cell IdentityImmune Cell PurityEpigenetic-based Quantitative PCRFlow CytometryAutomated Data AnalysisHematopoietic Cell SampleGenomic DNA IsolationBisulfite ConversionDNA PurificationParamagnetic Beads

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Pradhan, S. K., Guzman, J., Dargitz, C., Switalski, S., Landon, M., Olek, S., Samans, B., Hoffmueler, U., Lakshmipathy, U. Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR. J. Vis. Exp. (156), e60465, doi:10.3791/60465 (2020).

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