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Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
JoVE Revista
Genética
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JoVE Revista Genética
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
DOI:

09:21 min

September 13, 2019

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Capítulos

  • 00:04Título
  • 01:44Cell Isolation and Culture
  • 03:46Tumorsphere Derivation
  • 05:02Tumorsphere Preparation for Allograft Transplantation
  • 07:26Results: Tumorsphere Derivation and Transfection
  • 08:46Conclusion

Summary

Traducción Automática

This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.

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