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Handling and Assessment of Human Primary Prostate Organoid Culture
JoVE Revista
Bioingeniería
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JoVE Revista Bioingeniería
Handling and Assessment of Human Primary Prostate Organoid Culture

Handling and Assessment of Human Primary Prostate Organoid Culture

16,492 Views

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08:45 min

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January 17, 2019

DOI:

10.3791/59051-v

DOI:
10.3791/59051-v

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08:45 min
January 17, 2019

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17 Views
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1Department of Pathology,University of Illinois at Chicago, 2Departments of Urology, Physiology, and Biophysics,University of Illinois at Chicago, 3University of Illinois Cancer Center

Summary

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Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding, culture maintenance, recovery from matrix gel, morphologic quantification, embedding and sectioning, FFPE sectioning, whole-mount staining, and application of commercial assays are described.

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McCray, T., Richards, Z., Marsili, J., Prins, G. S., Nonn, L. Handling and Assessment of Human Primary Prostate Organoid Culture. J. Vis. Exp. (143), e59051, doi:10.3791/59051 (2019).

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