Dergi
/
Biyomühendislik
/
Handling and Assessment of Human Primary Prostate Organoid Culture
JoVE Journal
Biyomühendislik
This content is Free Access.
JoVE Journal Biyomühendislik
Handling and Assessment of Human Primary Prostate Organoid Culture

Handling and Assessment of Human Primary Prostate Organoid Culture

DOI:
10.3791/59051-v

•

08:45 min

•

January 17, 2019

•

, , , ,
1Patoloji Bölümü,University of Illinois at Chicago, 2Departments of Urology, Physiology, and Biophysics,University of Illinois at Chicago, 3University of Illinois Cancer Center

Bölümler

  • 00:04Başlık
  • 01:04Collection of Organoids from Matrix Gel
  • 02:09Formalin Fixation and Paraffin Embedding of Organoids for Histological Endpoints
  • 04:00Sectioning the Formalin Fixed Paraffin Embedded (FFPE) Histology Gel Blocks
  • 05:04Whole-mounting of Organoids for Immunofluorescent Staining
  • 06:35Results: Analysis of Human Primary Prostate Organoids
  • 07:56Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding, culture maintenance, recovery from matrix gel, morphologic quantification, embedding and sectioning, FFPE sectioning, whole-mount staining, and application of commercial assays are described.

Etiketler

Prostate OrganoidsIn Vitro CulturePrimary CellsMatrix GelNeutral ProteaseHistology EmbeddingPrecision MedicineDisease Modeling

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Read Article