Journal
/
Bio-ingénierie
/
Handling and Assessment of Human Primary Prostate Organoid Culture
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Handling and Assessment of Human Primary Prostate Organoid Culture

Handling and Assessment of Human Primary Prostate Organoid Culture

16,531 Views

•

08:45 min

•

January 17, 2019

DOI:

10.3791/59051-v

DOI:
10.3791/59051-v

•

08:45 min
January 17, 2019

•

17 Views
, , , ,
1Department of Pathology,University of Illinois at Chicago, 2Departments of Urology, Physiology, and Biophysics,University of Illinois at Chicago, 3University of Illinois Cancer Center

Summary

Automatically generated

Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding, culture maintenance, recovery from matrix gel, morphologic quantification, embedding and sectioning, FFPE sectioning, whole-mount staining, and application of commercial assays are described.

Explore More Videos

Prostate OrganoidsIn Vitro CulturePrimary CellsMatrix GelNeutral ProteaseHistology EmbeddingPrecision MedicineDisease Modeling

Read Article

Cite this Article

McCray, T., Richards, Z., Marsili, J., Prins, G. S., Nonn, L. Handling and Assessment of Human Primary Prostate Organoid Culture. J. Vis. Exp. (143), e59051, doi:10.3791/59051 (2019).

Download .ris file

Copy

Share Video

.

Copy