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Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
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Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Capítulos
- 00:04Título
- 01:13Plasmid Construction
- 03:25RNA Expression
- 04:52RNA Extraction and Purification
- 06:40RNA Analysis
- 07:32Results: Analysis of the Large-scale Production of Recombinant RNAs
- 09:19Conclusion
Here, we present a protocol to produce large amounts of recombinant RNA in Escherichia coli by co-expressing a chimeric RNA that contains the RNA of interest in a viroid scaffold and a plant tRNA ligase. The main product is a circular molecule that facilitates purification to homogeneity.
