JoVE Journal
Biochemistry
Biochemistry
需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。
章节
总结概括
Here, we present a protocol to produce large amounts of recombinant RNA in Escherichia coli by co-expressing a chimeric RNA that contains the RNA of interest in a viroid scaffold and a plant tRNA ligase. The main product is a circular molecule that facilitates purification to homogeneity.