ジャーナル
/
生化学
/
Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

DOI:
10.3791/58472-v

•

10:38 min

•

November 30, 2018

•

, ,
1Instituto de Biología Molecular y Celular de Plantas,Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Spain

章

  • 00:04標題
  • 01:13Plasmid Construction
  • 03:25RNA Expression
  • 04:52RNA Extraction and Purification
  • 06:40RNA Analysis
  • 07:32Results: Analysis of the Large-scale Production of Recombinant RNAs
  • 09:19Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol to produce large amounts of recombinant RNA in Escherichia coli by co-expressing a chimeric RNA that contains the RNA of interest in a viroid scaffold and a plant tRNA ligase. The main product is a circular molecule that facilitates purification to homogeneity.

タグ

Recombinant RNARNA ProductionCircular RNA ScaffoldViroidEscherichia ColiGibson AssemblyPCRRestriction EnzymeAgarose Gel ElectrophoresisMiniprep

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Detergent-assisted Reconstitution of Recombinant Drosophila Atlastin into Liposomes for Lipid-mixing Assays

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Read Article