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遺伝的定款生合成 L-ジヒドロキシフェニルアラニン (DOPA) とタンパク質接合への応用
Capítulos
- 00:04Título
- 00:29Culture Preparation
- 02:06Expression and Purification of GFP-E90DOPA by a Biosynthetic System
- 04:25Oligomerization of Purified GFP-E90DOPA and SDS-PAGE Analysis
- 05:47Conjugation of GFP-E90DOPA with an Alkyne Probe by SPOCQ, Purification of the Labeled GFP
- 07:10SDS-PAGE Analysis and Fluorescence Gel Scanning of Labeled GFP
- 08:21Results: Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine
- 10:01Conclusion
