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单细胞荧光显微镜定量 Efferocytosis 的研究
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
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单细胞荧光显微镜定量 Efferocytosis 的研究

DOI:
10.3791/58149-v

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06:15 min

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August 18, 2018

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, , ,
1Department of Microbiology and Immunology and the Center for Human Immunology,University of Western Ontario, 2Institute of Infection and Global Health,University of Liverpool, 3Department of Respiratory Research,Aintree University Hospital NHS Foundation Trust

Capítulos

  • 00:04Título
  • 00:50Quantifying Efferocytic Uptake and Dynamics Using a Fixed Cell Efferocytosis Assay and Inside-out Staining
  • 05:14Results: Overnight Culture of Jurkat Cells with Staurosporine Results in Apoptosis of Greater than 95% of Cells
  • 05:36Conclusion

Summary

Traducción Automática

Traducción Automática

Efferocytosis, 凋亡细胞的吞噬去除, 是需要保持动态平衡, 并由受体和信号通路, 以允许识别, 吞没和内化的凋亡细胞的促进。在此, 我们提出了一个荧光显微镜协议的量化 efferocytosis 和 efferocytic 信号通路的活动。

Tags

EfferocytosisSingle-cell Fluorescence MicroscopyApoptotic Cell UptakeMacrophagesJurkat CellsNHS-BiotinCell-tracking DyeFITC-conjugated StreptavidinQuantificationMicroscope Optimization

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