Journal
/
Inmunología y contagio
/
Kwantificering van Efferocytosis door eencellige fluorescentie microscopie
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Please note that all translations are automatically generated. Click here for the English version.

Kwantificering van Efferocytosis door eencellige fluorescentie microscopie

12,168 Views

•

06:15 min

•

August 18, 2018

DOI:

10.3791/58149-v

DOI:
10.3791/58149-v

•

06:15 min
August 18, 2018

•

15 Views
, , ,
1Department of Microbiology and Immunology and the Center for Human Immunology,University of Western Ontario, 2Institute of Infection and Global Health,University of Liverpool, 3Department of Respiratory Research,Aintree University Hospital NHS Foundation Trust

Summary

Automatically generated

Efferocytosis, de fagocytische verwijdering van apoptotic cellen, is nodig om de homeostase te handhaven en wordt vergemakkelijkt door de receptoren en signaalroutes waarmee voor de erkenning, de engulfment, en de internalisering van de apoptotic cellen. Hierin presenteren wij een fluorescentie microscopie protocol voor de kwantificering van efferocytosis en de activiteit van efferocytic signaalroutes.

Explore More Videos

EfferocytosisSingle-cell Fluorescence MicroscopyApoptotic Cell UptakeMacrophagesJurkat CellsNHS-BiotinCell-tracking DyeFITC-conjugated StreptavidinQuantificationMicroscope Optimization

Read Article

Cite this Article

Taruc, K., Yin, C., Wootton, D. G., Heit, B. Quantification of Efferocytosis by Single-cell Fluorescence Microscopy. J. Vis. Exp. (138), e58149, doi:10.3791/58149 (2018).

Download .ris file

Copy

Share Video

.

Copy