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Analisi trascrittomica unicellulare delle cellule Endocrine del pancreas del Mouse
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
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Analisi trascrittomica unicellulare delle cellule Endocrine del pancreas del Mouse

10,676 Views

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10:05 min

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September 30, 2018

DOI:

10.3791/58000-v

DOI:
10.3791/58000-v

•

10:05 min
September 30, 2018

•

23 Views
, , , , ,
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences,Peking University, 2Academy for Advanced Interdisciplinary Studies,Peking University, 3PKU-Tsinghua-NIBS Graduate Program,Peking University

Summary

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Descriviamo un metodo per l'isolamento di cellule endocrine da pancreas embrionale, neonatale e postnatale, seguite dal sequenziamento di RNA di cella singola. Questo metodo consente analisi dello sviluppo del pancreas endocrino lignaggio, eterogeneità e trascrittomica dinamiche di cella.

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Li, L., Yu, X., Zhang, Y., Feng, Y., Qiu, W., Xu, C. Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells. J. Vis. Exp. (139), e58000, doi:10.3791/58000 (2018).

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