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小鼠胰腺内分泌细胞的单细胞 Transcriptomic 分析
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Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
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小鼠胰腺内分泌细胞的单细胞 Transcriptomic 分析

10,676 Views

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10:05 min

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September 30, 2018

DOI:

10.3791/58000-v

DOI:
10.3791/58000-v

•

10:05 min
September 30, 2018

•

23 Views
, , , , ,
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences,Peking University, 2Academy for Advanced Interdisciplinary Studies,Peking University, 3PKU-Tsinghua-NIBS Graduate Program,Peking University

Summary

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我们描述了一种方法, 从胚胎, 新生儿和产后胰腺的内分泌细胞分离, 其次是单细胞 RNA 测序。这种方法可以分析胰腺内分泌谱系的发展, 细胞异质性和 transcriptomic 动力学。

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Cite this Article

Li, L., Yu, X., Zhang, Y., Feng, Y., Qiu, W., Xu, C. Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells. J. Vis. Exp. (139), e58000, doi:10.3791/58000 (2018).

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