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培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究

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Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

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培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究

DOI:

10.3791/57593-v

•

08:15 min

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May 02, 2018

•

Vera D. Rinaldi, Jordana C. Bloom, John C. Schimenti²

¹Department of Biomedical Sciences,Cornell University, ²Department of Molecular Biology and Genetics,Cornell University

Capítulos

00:04Título
00:50Preparation for Ovary Dissection and Culture
02:13Ovary Dissection and Organ Culture
03:34Tissue Fixation
04:12Whole Mount Immunofluorescence
05:42Ovary Clearing for Imaging
06:37Results: Immunofluorescence Imaging of Cleared Ovaries
07:23Conclusion

Summary

Traducción Automática

在这里, 我们提出一项协议, 以量化卵泡在培养卵巢小鼠没有连续切片。用全器官免疫荧光和组织清除, 用光学切片代替物理切片。这种样品制备和可视化方法保持了器官的完整性, 促进了特定细胞的自动定量。

Tags

Whole Mount Immunofluorescence Follicle Quantification Cultured Mouse Ovaries Reproductive Biology Three-dimensional Architecture Dissection Incubator Ovary Culture Media Imaging Automated Analysis

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