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Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

DOI:
10.3791/57548-v

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10:24 min

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May 15, 2018

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, ,
1Department of Neuroscience and Brain Technologies,Istituto Italiano di Tecnologia, 2Rehab Technologies,Istituto Italiano di Tecnologia

Capítulos

  • 00:04Título
  • 00:39Preparation of the MEA/Custom Chamber Assembly
  • 01:15Preparation of the Recording Bench
  • 02:52Preparation of the MEA Set-up
  • 04:39MEA Live Mapping
  • 06:33Recording and Electrical Modulation of the Epileptiform Activity
  • 08:49Results: Representative Experiment of Electrical Modulation of Ictogenesis Using Brain Slices Coupled to MEAs
  • 09:21Conclusion

Summary

Traducción Automática

Traducción Automática

We illustrate how to perform recording and electrical modulation of 4-aminopyridine-induced epileptiform activity in rodent brain slices using microelectrode arrays. A custom recording chamber maintains tissue viability throughout prolonged experimental sessions. Live electrode mapping and selection of stimulating pairs are performed by a custom graphical user interface.

Tags

Epileptiform ActivityRodent Brain SlicesMicroelectrode ArraysNeuromodulationEpilepsy TreatmentBrain CircuitsMEAACSF4APHippocampus-cortexBrain Slice PreparationPerfusionRecording ChamberHeatingAmplifier

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